S6 Ribosomal protein Recombinant antibody

S6 Ribosomal protein Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

6L9

Cat no : 80208-1-RR

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

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Validation Data Gallery

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  WB analysis using 80208-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80208-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 80208-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 80208-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using S6 Ribosomal protein antibody (80208-1-RR, Clone: 6L9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, RAW 264.7 cells, zebrafish tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, 4T1 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

80208-1-RR targets S6 Ribosomal protein in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• RRID: AB_2918876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

Species	Application	Title
mouse	WB	ACS Omega; ROS-responsive MSC-derived Exosome Mimetics Carrying MHY1485 Alleviate Renal Ischemia Reperfusion Injury through Multiple Mechanisms; Authors - Zhiyu Qian
human	WB	J Biol Chem; TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells; Authors - Suli Lv
human	WB	Biomark Res; Deciphering metabolic heterogeneity in retinoblastoma unravels the role of monocarboxylate transporter 1 in tumor progression; Authors - Junjie Tang