RPS6 Recombinant antibody

RPS6 Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse, zabrafish

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

6L9

Cat no : 80208-1-RR

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6



Tested Applications

Positive WB detected inLNCaP cells, RAW 264.7 cells, zebrafish tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, 4T1 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80208-1-RR targets RPS6 in WB, IF, ELISA applications and shows reactivity with Human, rat, mouse, zabrafish samples.

Tested Reactivity Human, rat, mouse, zabrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Protocols

Product Specific Protocols
WB protocol for RPS6 antibody 80208-1-RRDownload protocol
IF protocol for RPS6 antibody 80208-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols