S100A6 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

S100A6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10245-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 10245-1-AP (same clone as 10245-1-PBS)

  WB result of S100A6 antibody (10245-1-AP, 1:30000) with si-Control and si-S100A6 transfected HeLa cells.. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  WB analysis using 10245-1-AP (same clone as 10245-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 10245-1-AP (same clone as 10245-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 10245-1-AP (same clone as 10245-1-PBS)

  IP result of anti-S100A6 (IP:10245-1-AP, 4ug; Detection:10245-1-AP 1:5000) with A549 cells lysate 840 ug. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10245-1-AP (same clone as 10245-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A6 antibody (10245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10245-1-AP (same clone as 10245-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using S100A6 antibody 10245-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 10245-1-AP (same clone as 10245-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human S100A6 (10245-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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Product Information

10245-1-PBS targets S100A6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A6 fusion protein Ag0385
• Full Name: S100 calcium binding protein A6
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC001431
• Gene Symbol: S100A6
• Gene ID (NCBI): 6277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.