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  • Featured Product
  • KD/KO Validated

S100A6 Polyclonal antibody

S100A6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10245-1-AP

Synonyms

PRA, MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using S100A6 Polyclonal antibody (10245-1-AP)

    WB analysis of HeLa using 10245-1-AP

    WB result of S100A6 antibody (10245-1-AP, 1:30000) with si-Control and si-S100A6 transfected HeLa cells..

  • Western Blot (WB) analysis of various lysates using S100A6 Polyclonal antibody (10245-1-AP)

    WB analysis using 10245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using S100A6 Polyclonal antibody (10245-1-AP)

    WB analysis of A549 using 10245-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using S100A6 Polyclonal antibody (10245-1-AP)

    IP experiment of A549 using 10245-1-AP

    IP result of anti-S100A6 (IP:10245-1-AP, 4ug; Detection:10245-1-AP 1:5000) with A549 cells lysate 840 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human pancreas cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human pancreas cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human colon cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human colon cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using S100A6 Polyclonal antibody (10245-1-AP)

    IF Staining of MCF-7 using 10245-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A6 antibody (10245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using S100A6 Polyclonal antibody (10245-1-AP)

    IF Staining of MCF-7 using 10245-1-AP

    Immunofluorescent analysis of MCF-7 cells, using S100A6 antibody 10245-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Flow cytometry (FC) experiment of MCF-7 cells using S100A6 Polyclonal antibody (10245-1-AP)

    FC experiment of MCF-7 using 10245-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human S100A6 (10245-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10245-1-AP targets S100A6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A6 fusion protein Ag0385
Full Name S100 calcium binding protein A6
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC001431
Gene Symbol S100A6
Gene ID (NCBI) 6277
RRIDAB_2183801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.

Protocols

Product Specific Protocols
WB protocol for S100A6 antibody 10245-1-APDownload protocol
IHC protocol for S100A6 antibody 10245-1-APDownload protocol
IF protocol for S100A6 antibody 10245-1-APDownload protocol
IP protocol for S100A6 antibody 10245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Cellular architecture of human brain metastases.

Authors - Hugo Gonzalez
View Article
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
View Article
humanIHC,IF

Sci Transl Med

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.

Authors - Bradley N Smith
View Article
human,mouseWB,IHC,IF

Cancer Lett

S100A6 drives lymphatic metastasis of liver cancer via activation of the RAGE/NF-kB/VEGF-D pathway

Authors - TianYi Chen
View Article
humanIF

iScience

Lineage tracing reveals transient phenotypic adaptation of tubular cells during acute kidney injury

Authors - Marc Buse
View Article
humanIF

Cancer Commun (Lond)

Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.

Authors - Chaoji Shi
View Article