CoraLite®594-conjugated FSP1/S100A4 Polyclonal antibody

• Featured Product
• KD/KO Validated

FSP1/S100A4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16105

Synonyms

S100A4, FSP1, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein

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Validation Data Gallery

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  IF Staining of HeLa using CL594-16105

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 S100A4 antibody (CL594-16105) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-16105

  1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human S100A4 (CL594-16105) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL594-16105

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human S100A4 (CL594-16105) (red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells, Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-16105 targets FSP1/S100A4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FSP1/S100A4 fusion protein Ag9019
• Full Name: S100 calcium binding protein A4
• Calculated Molecular Weight: 101 aa, 12 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC016300
• Gene Symbol: S100A4
• Gene ID (NCBI): 6275
• RRID: AB_2919828
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C