Filter: AllWBIHCAffinity

WB analysis of A431 using 81017-1-RR (same clone as 81017-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 81017-1-RR (S100A10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81017-1-RR (S100A10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of mouse skin using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of mouse skin using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 81017-1-RR (same clone as 81017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 81017-1-RR
Biolayer interferometry (BLl) kinetic assays of 81017-1-RR against Human S100A10 were performed. The affinity constant is 24.5 nM.

S100A10 Recombinant antibody, PBS Only

S100A10 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 81017-1-PBS

Clone No.8G23

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG, ANX2L

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

81017-1-PBS targets S100A10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	S100A10 fusion protein Ag1779 Predict reactive species
Full Name	S100 calcium binding protein A10
Calculated Molecular Weight	11 kDa
Observed Molecular Weight	11 kDa
GenBank Accession Number	BC015973
Gene Symbol	S100A10
Gene ID (NCBI)	6281
RRID	AB_2918925
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P60903
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.