S100A10 Recombinant antibody

S100A10 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

8G23

Cat no : 81017-1-RR

Synonyms

Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG, ANX2L

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Validation Data Gallery

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  WB analysis of A431 using 81017-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 81017-1-RR (S100A10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81017-1-RR (S100A10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 81017-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 81017-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81017-1-RR against Human S100A10 were performed. The affinity constant is 24.5 nM.

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, U2OS cells, HaCaT cells, human lung tissue
• Positive IHC detected in: human colon tissue, mouse skin tissue, human pancreas cancer tissue, human lung cancer tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81017-1-RR targets S100A10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: S100A10 fusion protein Ag1779
• Full Name: S100 calcium binding protein A10
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC015973
• Gene Symbol: S100A10
• Gene ID (NCBI): 6281
• RRID: AB_2918925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.