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S100A10 Recombinant antibody

S100A10 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

8G23

Cat no : 81017-1-RR

Synonyms

Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG, ANX2L

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using S100A10 Recombinant antibody (81017-1-RR)

    WB analysis of A431 using 81017-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 81017-1-RR (S100A10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human lung cancer using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human colon using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81017-1-RR (S100A10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human lung cancer using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse skin using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse skin using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human pancreas cancer using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human pancreas cancer using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse lung using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse lung using 81017-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81017-1-RR

    Affinity and Kinetic Characterization of 81017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81017-1-RR against Human S100A10 were performed. The affinity constant is 24.5 nM.

Tested Applications

Positive WB detected inA431 cells, A549 cells, U2OS cells, HaCaT cells, human lung tissue
Positive IHC detected inhuman colon tissue, mouse skin tissue, human pancreas cancer tissue, human lung cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81017-1-RR targets S100A10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A10 fusion protein Ag1779
Full Name S100 calcium binding protein A10
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC015973
Gene Symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2918925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
WB protocol for S100A10 antibody 81017-1-RRDownload protocol
IHC protocol for S100A10 antibody 81017-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols