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Raptor Monoclonal antibody, PBS Only

Raptor Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8A12

Cat no : 68286-1-PBS

Synonyms

KIAA1303, raptor, RPTOR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Raptor Monoclonal antibody (68286-1-Ig)

    WB analysis using 68286-1-Ig (same clone as 68286-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68286-1-Ig (Raptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Raptor Monoclonal antibody (68286-1-Ig)

    IP experiment of HeLa using 68286-1-Ig (same clone as 68286-1-PBS)

    IP result of anti-Raptor (IP:68286-1-Ig, 4ug; Detection:68286-1-Ig 1:1000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68286-1-PBS targets Raptor in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Raptor fusion protein Ag30131
Full Name raptor
Calculated Molecular Weight 1335 aa, 149 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC136654
Gene Symbol Raptor
Gene ID (NCBI) 57521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.