Raptor Monoclonal antibody

Raptor Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B8A12

Cat no : 68286-1-Ig

Synonyms

KIAA1303, raptor, RPTOR



Tested Applications

Positive WB detected inMCF-7 cells, LNCaP cells, HEK-293 cells, MOLT-4 cells, Jurkat cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68286-1-Ig targets Raptor in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Raptor fusion protein Ag30131
Full Name raptor
Calculated Molecular Weight 1335 aa, 149 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC136654
Gene Symbol Raptor
Gene ID (NCBI) 57521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.

Protocols

Product Specific Protocols
WB protocol for Raptor antibody 68286-1-IgDownload protocol
IP protocol for Raptor antibody 68286-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells

Authors - Lixin He