Raptor Monoclonal antibody

Raptor Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B8A12

Cat no : 68286-1-Ig

Synonyms

KIAA1303, raptor, RPTOR

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Validation Data Gallery

• 

  WB analysis using 68286-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68286-1-Ig (Raptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 68286-1-Ig

  IP result of anti-Raptor (IP:68286-1-Ig, 4ug; Detection:68286-1-Ig 1:1000) with HeLa cells lysate 1800 ug.

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Tested Applications

• Positive WB detected in: MCF-7 cells, LNCaP cells, HEK-293 cells, MOLT-4 cells, Jurkat cells, HeLa cells, K-562 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68286-1-Ig targets Raptor in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Raptor fusion protein Ag30131
• Full Name: raptor
• Calculated Molecular Weight: 1335 aa, 149 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC136654
• Gene Symbol: Raptor
• Gene ID (NCBI): 57521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.

Publications

Species	Application	Title
human	WB	Mol Cell; Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells; Authors - Lixin He