CoraLite® Plus 750-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

Cat no : CL750-30000

Synonyms

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Validation Data Gallery

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  FC experiment of HepG2 using CL750-30000

  1x10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 750-conjugated Rabbit IgG control Polyclonal antibody (CL750-30000)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-30000 targets Rabbit IgG control in FC (Intra) applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: Rabbit IgG control
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.