Tested Applications
| Positive WB detected in | Control |
| Positive IP detected in | Control |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30000-0-AP targets Rabbit IgG control in WB, IHC, IP, CoIP, ChIP, RIP, IP-MS, ELISA, Blocking assay, Cell treatment applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Cited Reactivity | hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | Rabbit IgG control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2819035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Publications
What published studies show
The Rabbit IgG isotype control antibody (Cat# 30000-0-AP) has accumulated over 1,145 citations across high-impact journals including Nature, Cell, Cancer Cell, Signal Transduction and Targeted Therapy, Nature Communications, Molecular Cell, Autophagy, Advanced Science, Journal of Experimental & Clinical Cancer Research, Cancer Research, Cell Metabolism, Immunity, Science Advances, and Nucleic Acids Research. Citations span diverse research fields such as oncology, immunology, metabolism, autophagy, epigenetics, neuroscience, cardiology, and infectious disease, reflecting its broad utility as a negative control in WB, IP, ChIP, RIP, and IHC experiments.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification | ||
Signal Transduct Target Ther Krüppel-like factor 4 downregulation during esophageal cancer formation facilitates immune evasion and impairs immunotherapy | ||
Cancer Cell Epithelial cells activate fibroblasts to promote esophageal cancer development |
Reviews
What customers say
Users consistently rate this product 4–5 stars. It is most commonly used as a negative/isotype control for immunoprecipitation, with reviewers describing it as "perfect" and "very efficient." Several note it is highly concentrated and recommend using less than the typical amount. It also performs well in Western blot and neutralization experiments, with one user reporting good results at a 1:10,000 dilution. Cross-species applicability has been confirmed in human cell lines, Jurkat cells, glioblastoma lines, ARPE-19, and even C. elegans. Overall, customers find it reliable and recommend it as a dependable control reagent.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (11-20-2025) |
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Roy (Verified Customer) (10-07-2025) | Perfect control for IP
|
Celine (Verified Customer) (07-09-2025) | Works well as a negative control for immunoprecipitation
|
Siddharth (Verified Customer) (08-02-2023) | Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.
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Macarena (Verified Customer) (10-07-2022) | very concentrated. Excellent results. Deffinetly use less than you would normally use.
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Guo-rong (Verified Customer) (08-30-2022) | It works well as an antibody control in neutralization experiments
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Anita (Verified Customer) (01-04-2022) | Works well as a control for IP
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Kenneth (Verified Customer) (02-14-2019) | Great company with Great products. Highly recommended.
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Priyanka (Verified Customer) (12-18-2018) |
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Seongjin (Verified Customer) (12-14-2018) |
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