Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

and More (3)

Applications

WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

153

Reviews (6)

Cat no : 30000-0-AP

Synonyms

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin, RIGGCON

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Validation Data Gallery

• 

  WB analysis of Control using 30000-0-AP

  IP sample detected with different secondary antibodies. Normal Rabbit IgG (30000-0-AP) as control.

• 

  IP experiment of Control using 30000-0-AP

  IP sample detected with different secondary antibodies. Normal Rabbit IgG (30000-0-AP) as control.

• 

  FC experiment of Jurkat using 30000-0-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Rabbit IgG control (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Protein, Control
• Positive IP detected in: Control
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and N/A for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 24 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 37 publications below
• FC: See 3 publications below
• CoIP: See 15 publications below
• ChIP: See 20 publications below
• RIP: See 15 publications below

Product Information

30000-0-AP targets Rabbit IgG control in WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA applications and shows reactivity with samples.

• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: Rabbit IgG control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publications

Species	Application	Title
	IP	Drug Resist Updat; CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis; Authors - Jing Wu
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
	IP	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
	WB	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
	IP	ACS Nano; Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling; Authors - Bide Tong
mouse	CoIP	Adv Sci (Weinh); AQP4 Aggravates Cognitive Impairment in Sepsis-Associated Encephalopathy through Inhibiting Nav 1.6-Mediated Astrocyte Autophagy; Authors - Dan-Dan Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena (Verified Customer) (10-07-2022)

very concentrated. Excellent results. Deffinetly use less than you would normally use.

• Applications: Immunoprecipitation
• Cell Tissue Type: cell lines

FH

Guo-rong (Verified Customer) (08-30-2022)

It works well as an antibody control in neutralization experiments

• Applications: Cell culture
• Cell Tissue Type: ARPE-19

FH

Anita (Verified Customer) (01-04-2022)

Works well as a control for IP

• Applications: Immunoprecipitation
• Cell Tissue Type: C. elegans

FH

Kenneth (Verified Customer) (02-14-2019)

Great company with Great products. Highly recommended.

• Applications: Western Blot,

FH

Priyanka (Verified Customer) (12-18-2018)

• Applications: Western Blot, Immunofluorescence,

FH

Seongjin (Verified Customer) (12-14-2018)

• Applications: Immunoprecipitation,