Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

and More (1)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 30000-0-AP

Synonyms

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin, RIGGCON



Tested Applications

Positive WB detected inProtein, Control
Positive IP detected inControl

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and N/A for WB
Sample-dependent, check data in validation data gallery

Product Information

30000-0-AP targets Rabbit IgG control in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with samples.

Tested Reactivity
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N/A
Full Name Rabbit IgG control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publications

SpeciesApplicationTitle
IP

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
IP

Mol Cell

Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy.

Authors - Lulu Jiang
IP

J Extracell Vesicles

Secretion of pro-angiogenic extracellular vesicles during hypoxia is dependent on the autophagy-related protein GABARAPL1.

Authors - Tom G Keulers
ChIP

Nat Commun

Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors.

Authors - Karen R Mifsud
IP

Nat Commun

Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.

Authors - John K Vuong
ChIP

Nat Commun

Adaptation of the master antioxidant response connects metabolism, lifespan and feather development pathways in birds.

Authors - Gianni M Castiglione

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guo-rong (Verified Customer) (08-30-2022)

It works well as an antibody control in neutralization experiments

  • Applications: Cell culture
  • Cell Tissue Type: ARPE-19
FH

Anita (Verified Customer) (01-04-2022)

Works well as a control for IP

  • Applications: Immunoprecipitation
  • Cell Tissue Type: C. elegans
FH

Kenneth (Verified Customer) (02-14-2019)

Great company with Great products. Highly recommended.

  • Applications: Western Blot,
FH

Priyanka (Verified Customer) (12-18-2018)

  • Applications: Western Blot, Immunofluorescence,
FH

Seongjin (Verified Customer) (12-14-2018)

  • Applications: Immunoprecipitation,