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CoraLite®594-conjugated RRBP1 Polyclonal antibody

RRBP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-22015

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 180 kDa ribosome receptor homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated RRBP1 Polyclonal antibody (CL594-22015)

    IF Staining of HepG2 using CL594-22015

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated RRBP1 antibody (CL594-22015) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated RRBP1 Polyclonal antibody (CL594-22015)

    FC experiment of HeLa using CL594-22015

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RRBP1 (CL594-22015) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-22015 targets RRBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRBP1 fusion protein Ag16648
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Calculated Molecular Weight 1410 aa, 152 kDa
Observed Molecular Weight 180 kDa, 109 kDa
GenBank Accession NumberBC128577
Gene Symbol RRBP1
Gene ID (NCBI) 6238
RRIDAB_2919866
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

Protocols

Product Specific Protocols
IF protocol for CL594 RRBP1 antibody CL594-22015Download protocol
FC protocol for CL594 RRBP1 antibody CL594-22015Download protocol
Standard Protocols
Click here to view our Standard Protocols