Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse pancreas tissue, HepG2 cells|
|Positive IHC detected in||human lung cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||BxPC-3 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
22015-1-AP targets RRBP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RRBP1 fusion protein Ag16648|
|Full Name||ribosome binding protein 1 homolog 180kDa (dog)|
|Calculated molecular weight||1410 aa, 152 kDa|
|Observed molecular weight||180 kDa|
|GenBank accession number||BC128577|
|Gene ID (NCBI)||6238|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.
|Product Specific Protocols|
|WB protocol for RRBP1 antibody 22015-1-AP||Download protocol|
|IHC protocol for RRBP1 antibody 22015-1-AP||Download protocol|
|IF protocol for RRBP1 antibody 22015-1-AP||Download protocol|
|FC protocol for RRBP1 antibody 22015-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.
USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.
Acta Biochim Biophys Sin (Shanghai)
Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.
Cancer Manag Res
RRBP1 is highly expressed in prostate cancer and correlates with prognosis.
Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function
RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype