CoraLite®594-conjugated S6 Ribosomal protein Monoclonal antibody

S6 Ribosomal protein Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C3E10

Cat no : CL594-66886

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66886

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66886 (S6 Ribosomal protein antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66886 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: S6 Ribosomal protein fusion protein Ag6599
• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• RRID: AB_2883632
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.