RPS6 Monoclonal antibody

RPS6 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

1C3E10

Cat no : 66886-1-Ig

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6

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Validation Data Gallery

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  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (RPS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66886-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66886-1-Ig (RPS6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (RPS6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66886-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66886-1-Ig (RPS6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66886-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66886-1-Ig (RPS6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66886-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RPS6 (66886-1-Ig, Clone:1C3E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, NIH/3T3 cells, rat liver tissue, HEK-293 cells, Jurkat cells
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

66886-1-Ig targets RPS6 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPS6 fusion protein Ag6599
• Full Name: ribosomal protein S6
• Calculated molecular weight: 29 kDa
• Observed molecular weight: 29-32 kDa
• GenBank accession number: BC000524
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

Species	Application	Title
human	WB	Genome Biol; A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.; Authors - Sooncheol Lee
human	WB,FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	WB	BMC Cancer; Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer.; Authors - Yaoxiang Tang
rat	WB	Endocrinology; Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-Mediated Effects on mTORC1 and mTORC2.; Authors - Yan Fu
human	WB	Sci Rep; YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma.; Authors - Yuting Zhan
mouse	WB	Ecotoxicol Environ Saf; Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.; Authors - Yifan Hong

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C