Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, NIH/3T3 cells, rat liver tissue, HEEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, HEK-293 cells |
| Positive IHC detected in | human placenta tissue, mouse pancreas tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66886-1-Ig targets S6 Ribosomal protein in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6599 Product name: Recombinant human RPS6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-249 aa of BC000524 Sequence: MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK Predict reactive species |
| Full Name | ribosomal protein S6 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 29-32 kDa |
| GenBank Accession Number | BC000524 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_2882218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for S6 Ribosomal protein antibody 66886-1-Ig | Download protocol |
| IF protocol for S6 Ribosomal protein antibody 66886-1-Ig | Download protocol |
| IHC protocol for S6 Ribosomal protein antibody 66886-1-Ig | Download protocol |
| WB protocol for S6 Ribosomal protein antibody 66886-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
S6 Ribosomal protein Monoclonal antibody (1C3E10), catalog number 66886-1-Ig, has 38 citations spanning journals including Nature Communications, Molecular Cell, Cell Reports, Autophagy, Advanced Science, Cancer Letters, and Genome Biology. Research fields encompass mTOR signaling, ribosome biology, cancer (hepatocellular, ovarian, gastric, lung, and others), translation regulation, stress granules, metabolism, neuroscience, immunology, virology, muscle physiology, bone biology, and reproductive health.
| Species | Application | Title |
|---|---|---|
Autophagy PSAT1 inhibits mTORC1 activation by preventing Rag heterodimer formation in lung adenocarcinoma | ||
Autophagy The ubiquitination of CD274 at MERCs enhances the anti-tumor immunity of cervical cancer. | ||
Nat Commun Tumor-derived GDF15 induces CCN3⁺ Schwann cells to promote cancer pain in pancreatic cancer. | ||
Metabolism Anti-sarcopenic effects of active vitamin D through modulation of anabolic and catabolic signaling pathways in human skeletal muscle: A randomized controlled trial | ||
Genes Dis Non-canonical role of "S6K1-SGK1" pathway in neuronal necroptosis following traumatic brain injury. |
Reviews
What customers say
Users report this antibody performs well in Western blot and immunofluorescence. Two reviewers gave 5-star ratings: one confirmed reliable WB (1/1000) and IF (1/100) performance on HeLa cells, while another achieved clear RPS6 downregulation detection at 1:10000 in Neuro2a cells. One reviewer gave 3 stars, noting that detection on total protein extracts from HCT116 cells was challenging, requiring at least 50 µg of protein and longer exposure times. Overall, the antibody is considered effective with appropriate optimization of loading and exposure conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Céline (Verified Customer) (04-13-2026) | Antibody that works well in Western blot 1/1000 in 2,5% milk in TBS and 0,05%Tween. It also works in immunofluorescence at 1/100.
|
Vandana (Verified Customer) (02-09-2026) | Worked well; Clear detection of RPS6 downregulation in siRNA knockdown cells
 |
Morgane (Verified Customer) (09-15-2025) | Quite hard to detect on total protein extract, needs at least 50µg protein and longer exposure
 |
