Ribophorin I Recombinant antibody, PBS Only (Capture/Detector)

Ribophorin I Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240044D4

Cat no : 83250-2-PBS

Synonyms

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit

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Validation Data Gallery

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  WB analysis using 83250-2-RR (same clone as 83250-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00231-3

  Sandwich ELISA standard curve of MP00231-3, Human Ribophorin I Recombinant Matched Antibody Pair - PBS only. 83250-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3576. 83250-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00231-3

  The mean Ribophorin I concentration was determined to be 145.4 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 11.0 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load.

• 

  IHC staining of human stomach cancer using 83250-2-RR (same clone as 83250-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83250-2-RR (same clone as 83250-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00231-2

  Cytometric bead array standard curve of MP00231-2, RPN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83250-2-PBS. Detection antibody: 83250-1-PBS. Standard: Ag3576. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 83250-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83250-2-PBS against Human Ribophorin I was performed. The affinity constant is 1.69 nM.

• 

  Affinity and Kinetic Characterization of 83250-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

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Product Information

83250-2-PBS targets Ribophorin I as part of a matched antibody pair:

MP00231-2: 83250-2-PBS capture and 83250-1-PBS detection (validated in Cytometric bead array)

MP00231-3: 83250-1-PBS capture and 83250-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Ribophorin I fusion protein Ag3576
• Full Name: ribophorin I
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 66-69 kDa
• GenBank Accession Number: BC010839
• Gene Symbol: RPN1
• Gene ID (NCBI): 6184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.