• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Ribophorin I Recombinant antibody

RPN1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240044D4

Cat no : 83250-2-RR

Synonyms

RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, 240044D4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RPN1 Recombinant antibody (83250-2-RR)

    WB analysis using 83250-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using RPN1 Recombinant antibody (83250-2-RR)

    FC experiment of HeLa using 83250-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83250-2-RR

    Affinity and Kinetic Characterization of 83250-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83250-2-RR targets RPN1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPN1 fusion protein Ag3576
Full Name ribophorin I
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 66-69 kDa
GenBank Accession NumberBC010839
Gene Symbol RPN1
Gene ID (NCBI) 6184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPN1 antibody 83250-2-RRDownload protocol
FC protocol for RPN1 antibody 83250-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols