RPN1 Recombinant antibody

RPN1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240044D4

Cat no : 83250-2-RR

Synonyms

DKFZp686B16177, hrd2, OST1, RBPH1, Ribophorin 1, ribophorin I, RPN I, RPN1

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Validation Data Gallery

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  WB analysis using 83250-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity experiments of 83250-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83250-2-RR targets RPN1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPN1 fusion protein Ag3576
• Full Name: ribophorin I
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 66-69 kDa
• GenBank Accession Number: BC010839
• Gene Symbol: RPN1
• Gene ID (NCBI): 6184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.