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CoraLite® Plus 488-conjugated RNASEL Polyclonal antibody

RNASEL Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22577

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RNASEL Polyclonal an (CL488-22577)

    IF Staining of HepG2 using CL488-22577

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RNASEL antibody (CL488-22577) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-22577 targets RNASEL in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEL fusion protein Ag17409
Full Name ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
Calculated Molecular Weight 741 aa, 84 kDa
Observed Molecular Weight 84 kDa; 73 kDa
GenBank Accession NumberBC115697
Gene Symbol RNASEL
Gene ID (NCBI) 6041
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RNASEL antibody CL488-22577Download protocol
Standard Protocols
Click here to view our Standard Protocols