CoraLite® Plus 488-conjugated RCHY1 Monoclonal antibody

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  IF Staining of HeLa using CL488-67856

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RCHY1 antibody (CL488-67856, Clone: 2C11C11 ) at dilution of 1:200.

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  IF Staining of A549 using CL488-67856

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 RCHY1 antibody (CL488-67856, Clone: 2C11C11 ) at dilution of 1:200.

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  FC experiment of PC-12 using CL488-67856

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RCHY1 (CL488-67856, Clone:2C11C11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

RCHY1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-67856

Clone No.2C11C11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

PIRH2, Androgen receptor N-terminal-interacting protein, ARNIP, CHIMP, CH-rich-interacting match with PLAG1

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Tested Applications

• Positive IF/ICC detected in: A549 cells, HeLa cells
• Positive FC (Intra) detected in: PC-12 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67856 targets RCHY1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4942

  Product name: Recombinant human RCHY1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-261 aa of BC047393

  Sequence: MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSQQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ

• Full Name: ring finger and CHY zinc finger domain containing 1
• Calculated Molecular Weight: 261 aa, 30 kDa
• GenBank Accession Number: BC047393
• Gene Symbol: RCHY1
• Gene ID (NCBI): 25898
• RRID: AB_2923805
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96PM5
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.