RCHY1 Monoclonal antibody

• Featured Product
• KD/KO Validated

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2C11C11

Cat no : 67856-1-Ig

Synonyms

ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

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Validation Data Gallery

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  WB analysis of HEK-293 using 67856-1-Ig

  WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells.

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  WB analysis using 67856-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human breast cancer using 67856-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67856-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 67856-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67856-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, Daudi cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

67856-1-Ig targets RCHY1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RCHY1 fusion protein Ag4942
• Full Name: ring finger and CHY zinc finger domain containing 1
• Calculated Molecular Weight: 261 aa, 30 kDa
• GenBank Accession Number: BC047393
• Gene Symbol: RCHY1
• Gene ID (NCBI): 25898
• RRID: AB_2918614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

Publications

Species	Application	Title
human	WB	iScience; An AKT1-and TRIM21-mediated phosphodegron controls proteasomal degradation of HuR enabling cell survival under heat shock; Authors - Sharanya Nag ; KD Validated