CoraLite® Plus 488-conjugated RBP5 Monoclonal antibody

RBP5 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G7E2

Cat no : CL488-68126

Synonyms

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-68126

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

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  IF Staining of A431 using CL488-68126

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68126 targets RBP5 in IF/ICC applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBP5 fusion protein Ag24562
• Full Name: retinol binding protein 5, cellular
• Calculated Molecular Weight: 135 aa, 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC029355
• Gene Symbol: RBP5
• Gene ID (NCBI): 83758
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).