RBP5 Monoclonal antibody

RBP5 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G7E2

Cat no : 68126-1-Ig

Synonyms

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

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Validation Data Gallery

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  WB analysis of pig kidney using 68126-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 68126-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68126-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68126-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of A431 using 68126-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: pig kidney tissue, pig liver tissue, pig liver tisse
• Positive IF/ICC detected in: HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68126-1-Ig targets RBP5 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBP5 fusion protein Ag24562
• Full Name: retinol binding protein 5, cellular
• Calculated Molecular Weight: 135 aa, 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC029355
• Gene Symbol: RBP5
• Gene ID (NCBI): 83758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).