CoraLite® Plus 488-conjugated RBP4 Monoclonal antibody

RBP4 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D12B11

Cat no : CL488-66104

Synonyms

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-66104

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 RBP4 antibody (CL488-66104, Clone: 1D12B11 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-66104

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RBP4 antibody (CL488-66104, Clone: 1D12B11 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66104 targets RBP4 in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBP4 fusion protein Ag19295
• Full Name: retinol binding protein 4, plasma
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC020633
• Gene Symbol: RBP4
• Gene ID (NCBI): 5950
• RRID: AB_2919286
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)