RBP4 Monoclonal antibody

RBP4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D12B11

Cat no : 66104-1-Ig

Synonyms

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

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Validation Data Gallery

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  WB analysis of human plasma using 66104-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66104-1-Ig (RBP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IF Staining of human liver cancer using 66104-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66104-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Human Blood, tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66104-1-Ig targets RBP4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBP4 fusion protein Ag19295
• Full Name: retinol binding protein 4, plasma
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC020633
• Gene Symbol: RBP4
• Gene ID (NCBI): 5950
• RRID: AB_2881503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)