CoraLite®594-conjugated RBM4 Polyclonal antibody

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• KD/KO Validated

RBM4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11614

Synonyms

RNA binding protein 4, RNA binding motif protein 4a, RNA binding motif protein 4, RBM4A, Lark homolog

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Validation Data Gallery

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  IF Staining of HeLa using CL594-11614

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

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  IF Staining of HepG2 using CL594-11614

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

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  FC experiment of HeLa using CL594-11614

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM4 (CL594-11614) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11614 targets RBM4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM4 fusion protein Ag2190
• Full Name: RNA binding motif protein 4
• Calculated Molecular Weight: 40 kDa
• GenBank Accession Number: BC021120
• Gene Symbol: RBM4
• Gene ID (NCBI): 5936
• RRID: AB_2919770
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C