RBM4 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBM4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, RIP, PLA, IHC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 11614-1-AP

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

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Validation Data Gallery

• 

  WB analysis of HeLa using 11614-1-AP

  WB result of RBM4 antibody (11614-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

• 

  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11614-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11614-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11614-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11614-1-AP

  IP result of anti-RBM4 (IP:11614-1-AP, 3ug; Detection:11614-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11614-1-AP (RBM4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, human heart tissue, human kidney tissue, HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human gliomas tissue, human lung cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• RIP: See 1 publications below

Product Information

11614-1-AP targets RBM4 in WB, IP, IF, FC, RIP, PLA, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM4 fusion protein Ag2190
• Full Name: RNA binding motif protein 4
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC021120
• Gene Symbol: RBM4
• Gene ID (NCBI): 5936
• RRID: AB_2176095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

Publications

Species	Application	Title
human	WB	Nature; An oxygen-regulated switch in the protein synthesis machinery.; Authors - Uniacke James J
human	WB, IHC	Cancer Cell; The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.; Authors - Yang Wang ; KD Validated
human	WB	Nucleic Acids Res; Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein.; Authors - Evelyne Manet
human	WB	Nat Commun; A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation.; Authors - Yangfan Qi
human	WB	Int J Mol Sci; G-Quadruplex Regulation of VEGFA mRNA Translation by RBM4.; Authors - Kangkang Niu ; KD Validated
human	IHC	Sci Rep; Prognostic value of decreased expression of RBM4 in human gastric cancer.; Authors - Hongmei Yong