CoraLite®555-conjugated RBM4 Monoclonal antibody

• Featured Product
• KD/KO Validated

RBM4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3H3F12

Cat no : CL555-60292

Synonyms

DKFZp547K0918, hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

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Validation Data Gallery

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  IF Staining of HeLa using CL555-60292

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 RBM4 antibody (CL555-60292, Clone: 3H3F12 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60292 targets RBM4 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBM4 fusion protein Ag2190
• Full Name: RNA binding motif protein 4
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC021120
• Gene Symbol: RBM4
• Gene ID (NCBI): 5936
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.