RBM4 Monoclonal antibody

• Featured Product
• KD/KO Validated

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H3F12

Cat no : 60292-1-Ig

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

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Validation Data Gallery

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  WB analysis of HeLa using 60292-1-Ig

  WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

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  WB analysis using 60292-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 60292-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 60292-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach using 60292-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 60292-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 60292-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 60292-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, fetal human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human cervical cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60292-1-Ig targets RBM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBM4 fusion protein Ag2190
• Full Name: RNA binding motif protein 4
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC021120
• Gene Symbol: RBM4
• Gene ID (NCBI): 5936
• RRID: AB_2881407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.