CoraLite®594-conjugated RBAP48 Monoclonal antibody

RBAP48 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5C4D6

Cat no : CL594-66060

Synonyms

RBBP4, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48, CAF-I 48 kDa subunit

---

Validation Data Gallery

• 

  IF Staining of A549 using CL594-66060

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594-conjugated RBAP48 antibody (CL594-66060, Clone: 5C4D6 ) at dilution of 1:200, CL488-Phalloidin (green).

• 

  FC experiment of A549 using CL594-66060

  1X10^6 A549 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human RBAP48 (CL594-66060, Clone:5C4D6) (red), or 0.2 ug Control Antibody (CL594-66360-3, Clone: ) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66060 targets RBAP48 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBAP48 fusion protein Ag6196
• Full Name: retinoblastoma binding protein 4
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC053904
• Gene Symbol: RBBP4
• Gene ID (NCBI): 5928
• RRID: AB_2883497
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011