RAE1 Recombinant antibody, PBS Only (Capture/Detector)

RAE1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240861E12

Cat no : 83712-1-PBS

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

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Validation Data Gallery

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  WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00683-1

  Cytometric bead array standard curve of MP00683-1, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-3-PBS. Detection antibody: 83712-1-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP00683-3

  Cytometric bead array standard curve of MP00683-3, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-1-PBS. Detection antibody: 83712-2-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83712-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

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Product Information

83712-1-PBS targets RAE1 as part of a matched antibody pair:

MP00683-1: 83712-3-PBS capture and 83712-1-PBS detection (validated in Cytometric bead array)

MP00683-3: 83712-1-PBS capture and 83712-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAE1 fusion protein Ag12102
• Full Name: RAE1 RNA export 1 homolog (S. pombe)
• Calculated Molecular Weight: 368 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC103754
• Gene Symbol: RAE1
• Gene ID (NCBI): 8480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)