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RAE1 Recombinant antibody

RAE1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240861E12

Cat no : 83712-1-RR

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAE1 Recombinant antibody (83712-1-RR)

    WB analysis using 83712-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using RAE1 Recombinant antibody (83712-1-RR)

    FC experiment of HepG2 using 83712-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83712-1-RR

    Affinity and Kinetic Characterization of 83712-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, HeLa cells, HEK-293 cells, BxPC-3 cells, mouse brain tissue, mouse thymus tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83712-1-RR is RAE1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAE1 fusion protein Ag12102
Full Name RAE1 RNA export 1 homolog (S. pombe)
Calculated Molecular Weight 368 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC103754
Gene Symbol RAE1
Gene ID (NCBI) 8480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

Protocols

Product Specific Protocols
WB protocol for RAE1 antibody 83712-1-RRDownload protocol
FC protocol for RAE1 antibody 83712-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols