RAD51C Recombinant antibody, PBS Only

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  WB analysis using 84537-5-RR (same clone as 84537-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84537-5-RR (RAD51C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84537-5-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 84537-5-RR (same clone as 84537-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RAD51C Recombinant antibody (84537-5-RR, Clone:241971A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84537-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84537-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84537-5-RR against Human RAD51C were performed. The affinity constant is below 1 pM.

RAD51C {ptg:Brand} Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84537-5-PBS

Clone No.241971A11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

241971A11

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Product Information

84537-5-PBS targets RAD51C in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAD51C fusion protein Ag35070
• Full Name: RAD51 homolog C (S. cerevisiae)
• Calculated Molecular Weight: 376 aa, 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC107753
• Gene Symbol: RAD51C
• Gene ID (NCBI): 5889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43502
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).