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  • Western Blot (WB) analysis of various lysates using RAD51C Recombinant antibody (84537-5-RR)

    WB analysis using 84537-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84537-5-RR (RAD51C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using RAD51C Recombinant antibody (84537-5-RR)

    FC experiment of MCF-7 using 84537-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RAD51C Recombinant antibody (84537-5-RR, Clone:241971A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84537-5-RR

    Affinity and Kinetic Characterization of 84537-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84537-5-RR against Human RAD51C were performed. The affinity constant is below 1 pM.

RAD51C Recombinant antibody

RAD51C Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84537-5-RR
Clone No.241971A11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

241971A11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, MDA-MB-231 cells, MCF-7 cells, T-47D cells, mouse testis tissue
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84537-5-RR targets RAD51C in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAD51C fusion protein Ag35070 Predict reactive species
Full Name RAD51 homolog C (S. cerevisiae)
Calculated Molecular Weight 376 aa, 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC107753
Gene Symbol RAD51C
Gene ID (NCBI) 5889
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43502
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).

Protocols

Product Specific Protocols
WB protocol for RAD51C antibody 84537-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols