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CoraLite® Plus 488-conjugated RAD23B Monoclonal antibody

RAD23B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E6G9

Cat no : CL488-67988

Synonyms

XP-C repair-complementing complex 58 kDa protein, p58, HR23B, HHR23B, 2E6G9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RAD23B Monoclonal an (CL488-67988)

    IF Staining of HeLa using CL488-67988

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RAD23B antibody (CL488-67988, Clone: 2E6G9 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated RAD23B Monoclonal an (CL488-67988)

    FC experiment of HeLa using CL488-67988

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAD23B (CL488-67988, Clone:2E6G9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67988 targets RAD23B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD23B fusion protein Ag28658
Full Name RAD23 homolog B (S. cerevisiae)
Calculated Molecular Weight 409 aa, 43 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC020973
Gene Symbol RAD23B
Gene ID (NCBI) 5887
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
FC protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
Standard Protocols
Click here to view our Standard Protocols