Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 67988-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 67988-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 67988-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD23B antibody (67988-1-Ig, Clone: 2E6G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD23B antibody (67988-1-Ig, Clone: 2E6G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
LNCaP cells, pig brain tissue, Hela cells, HEK-293 cells, Huh-7 cells, human platelet cells, rat brain tissue, mouse brain tissue
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67988-1-Ig targets RAD23B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).
Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 67988-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 67988-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 67988-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 67988-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD23B antibody (67988-1-Ig, Clone: 2E6G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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