RACGAP1 Recombinant antibody, PBS Only

RACGAP1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5

Cat no : 82788-1-PBS

Synonyms

HsCYK4, HsCYK 4, Gap1, GAP, CYK4

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Validation Data Gallery

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  WB analysis using 82788-1-RR (same clone as 82788-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82788-1-RR (RACGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 82788-1-RR (same clone as 82788-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82788-1-RR (RACGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82788-1-RR (same clone as 82788-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RACGAP1 antibody (82788-1-RR, Clone: 2D5 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82788-1-RR (same clone as 82788-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RACGAP1 Recombinant antibody (82788-1-RR, Clone:2D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82788-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82788-1-RR against Human RACGAP1 were performed. The affinity constant is 0.164 nM.

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Product Information

82788-1-PBS targets RACGAP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RACGAP1 fusion protein Ag4676
• Full Name: Rac GTPase activating protein 1
• Calculated Molecular Weight: 632 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC032754
• Gene Symbol: RACGAP1
• Gene ID (NCBI): 29127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.