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RACGAP1 Recombinant antibody

RACGAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D5

Cat no : 82788-1-RR

Synonyms

HsCYK4, HsCYK 4, Gap1, GAP, CYK4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RACGAP1 Recombinant antibody (82788-1-RR)

    WB analysis using 82788-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82788-1-RR (RACGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RACGAP1 Recombinant antibody (82788-1-RR)

    IHC staining of human skin cancer using 82788-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82788-1-RR (RACGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RACGAP1 Recombinant antibody (82788-1-RR)

    IF Staining of U2OS using 82788-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RACGAP1 antibody (82788-1-RR, Clone: 2D5 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using RACGAP1 Recombinant antibody (82788-1-RR)

    FC experiment of HeLa using 82788-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RACGAP1 Recombinant antibody (82788-1-RR, Clone:2D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82788-1-RR

    Affinity and Kinetic Characterization of 82788-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82788-1-RR against Human RACGAP1 were performed. The affinity constant is 0.164 nM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, U2OS cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82788-1-RR targets RACGAP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RACGAP1 fusion protein Ag4676
Full Name Rac GTPase activating protein 1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032754
Gene Symbol RACGAP1
Gene ID (NCBI) 29127
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Protocols

Product Specific Protocols
WB protocol for RACGAP1 antibody 82788-1-RRDownload protocol
IHC protocol for RACGAP1 antibody 82788-1-RRDownload protocol
IF protocol for RACGAP1 antibody 82788-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols