RAB33A Monoclonal antibody, PBS Only

RAB33A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7F11

Cat no : 68389-1-PBS

Synonyms

RAB33A, RabS10, Ras related protein Rab 33A, Small GTP binding protein S10

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Validation Data Gallery

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  WB analysis using 68389-1-Ig (same clone as 68389-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 68389-1-Ig (same clone as 68389-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 68389-1-Ig (same clone as 68389-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using RAB33A antibody (68389-1-Ig, Clone: 1C7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

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Product Information

68389-1-PBS targets RAB33A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

• Tested Reactivity: Human, rat, mouse, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB33A fusion protein Ag33188
• Full Name: RAB33A, member RAS oncogene family
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: BC009996
• Gene Symbol: RAB33A
• Gene ID (NCBI): 9363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).