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RAB33A Monoclonal antibody

RAB33A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7F11

Cat no : 68389-1-Ig

Synonyms

RAB33A, RabS10, Ras related protein Rab 33A, Small GTP binding protein S10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB33A Monoclonal antibody (68389-1-Ig)

    WB analysis using 68389-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of SH-SY5Y cells using RAB33A Monoclonal antibody (68389-1-Ig)

    WB analysis of SH-SY5Y using 68389-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using RAB33A Monoclonal antibody (68389-1-Ig)

    IF Staining of Neuro-2a using 68389-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using RAB33A antibody (68389-1-Ig, Clone: 1C7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, SH-SY5Y cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

68389-1-Ig targets RAB33A in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

Tested Reactivity Human, rat, mouse, pig, rabbit
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB33A fusion protein Ag33188
Full Name RAB33A, member RAS oncogene family
Calculated Molecular Weight 27 kDa
GenBank Accession NumberBC009996
Gene Symbol RAB33A
Gene ID (NCBI) 9363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).

Protocols

Product Specific Protocols
WB protocol for RAB33A antibody 68389-1-IgDownload protocol
IF protocol for RAB33A antibody 68389-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Neurosci

Rab6-Mediated Polarized Transport of Synaptic Vesicle Precursors Is Essential for the Establishment of Neuronal Polarity and Brain Formation

Authors - Yu Zhang
View Article