Various lysates were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of SH-SY5Y using 68389-1-Ig
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF Staining of Neuro-2a using 68389-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using RAB33A antibody (68389-1-Ig, Clone: 1C7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using RAB33A antibody (68389-1-Ig, Clone: 1C7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).
Various lysates were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of SH-SY5Y using 68389-1-Ig
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of Neuro-2a using 68389-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using RAB33A antibody (68389-1-Ig, Clone: 1C7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RAB33A Monoclonal antibody
Catalog Number
68389-1-Ig
Citations
1
Dilutions
WB : 1:2000-1:10000 IF/ICC : 1:200-1:800
Applications
WB, IF/ICC, ELISA
Reactivity
human, rat, mouse, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
