CoraLite® Plus 488-conjugated RAB11A/B Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB11A/B Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15903

Synonyms

Rab 11, RAB11, RAB11A, Ras related protein Rab 11A, YL8

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-15903

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 RAB11A/B antibody (CL488-15903) at dilution of 1:200.

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  IF Staining of U2OS using CL488-15903

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 RAB11A/B antibody (CL488-15903) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells, U2OS cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15903 targets RAB11A/B in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB11A/B fusion protein Ag8742
• Full Name: RAB11A, member RAS oncogene family
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC013348
• Gene Symbol: RAB11A
• Gene ID (NCBI): 8766
• RRID: AB_2919124
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).