Pyruvate Carboxylase Monoclonal antibody, PBS Only

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• KD/KO Validated

Pyruvate Carboxylase Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat , mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F5D1

Cat no : 66615-1-PBS

Synonyms

PC, PCB, pyruvate carboxykinase, pyruvate carboxylase, Pyruvic carboxylase

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Validation Data Gallery

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  WB analysis using 66615-1-Ig (same clone as 66615-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66615-1-Ig (Pyruvate Carboxylase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66615-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66615-1-Ig (same clone as 66615-1-PBS)

  WB result of Pyruvate Carboxylase antibody (66615-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pyruvate Carboxylase transfected HepG2 cells. This data was developed using the same antibody clone with 66615-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66615-1-Ig (same clone as 66615-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66615-1-Ig (Pyruvate Carboxylase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66615-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66615-1-Ig (same clone as 66615-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66615-1-Ig (Pyruvate Carboxylase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66615-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66615-1-PBS targets Pyruvate Carboxylase in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat , mouse samples.

• Tested Reactivity: Human, Rat , mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Pyruvate Carboxylase fusion protein Ag8846
• Full Name: pyruvate carboxylase
• Calculated Molecular Weight: 1178 aa, 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC011617
• Gene Symbol: PC
• Gene ID (NCBI): 5091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.