Pyruvate Carboxylase Monoclonal antibody

• Featured Product
• KD/KO Validated

Pyruvate Carboxylase Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat , mouse and More (1)

Applications

WB, IHC, RIP, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

4F5D1

Cat no : 66615-1-Ig

Synonyms

PC, PCB, pyruvate carboxykinase, pyruvate carboxylase, Pyruvic carboxylase

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Validation Data Gallery

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  WB analysis of HepG2 using 66615-1-Ig

  WB result of Pyruvate Carboxylase antibody (66615-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pyruvate Carboxylase transfected HepG2 cells.

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  WB analysis using 66615-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66615-1-Ig (Pyruvate Carboxylase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66615-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66615-1-Ig (Pyruvate Carboxylase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66615-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66615-1-Ig (Pyruvate Carboxylase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HSC-T6 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• RIP: See 1 publications below

Product Information

66615-1-Ig targets Pyruvate Carboxylase in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Rat , mouse samples.

• Tested Reactivity: Human, Rat , mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Pyruvate Carboxylase fusion protein Ag8846
• Full Name: pyruvate carboxylase
• Calculated Molecular Weight: 1178 aa, 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC011617
• Gene Symbol: Pyruvate Carboxylase
• Gene ID (NCBI): 5091
• RRID: AB_2881975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human	WB,RIP	Front Oncol; piR-hsa-211106 Inhibits the Progression of Lung Adenocarcinoma Through Pyruvate Carboxylase and Enhances Chemotherapy Sensitivity.; Authors - Yongmei Liu
human	WB	Elife; Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells; Authors - Madeleine L Hart ; KO Validated