CoraLite® Plus 488-conjugated Podocalyxin Polyclonal antibody

• Featured Product
• KD/KO Validated

Podocalyxin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18150

Synonyms

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60

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Validation Data Gallery

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  IF Staining of human kidney using CL488-18150

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Podocalyxin antibody (CL488-18150) at dilution of 1:200.

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  IF Staining of human kidney using CL488-18150

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Podocalyxin antibody (CL488-18150) at dilution of 1:200.

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  FC experiment of HeLa using CL488-18150

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Podocalyxin (CL488-18150) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human kidney tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-18150 is Podocalyxin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Podocalyxin fusion protein Ag12844
• Full Name: podocalyxin-like
• Calculated Molecular Weight: 526 aa, 55 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC093730
• Gene Symbol: PODXL
• Gene ID (NCBI): 5420
• RRID: AB_2919156
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C