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CoraLite® Plus 488-conjugated Podocalyxin Polyclonal antibody
Podocalyxin Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-18150
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-18150 targets Podocalyxin in IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Podocalyxin fusion protein Ag12844 |
| Full Name | podocalyxin-like |
| Calculated Molecular Weight | 526 aa, 55 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC093730 |
| Gene Symbol | Podocalyxin |
| Gene ID (NCBI) | 5420 |
| RRID | AB_2919156 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It is Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in many cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Podocalyxin antibody CL488-18150 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
