CoraLite® Plus 488-conjugated Plasminogen Monoclonal antibody

Plasminogen Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F6A11

Cat no : CL488-66399

Synonyms

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin, 1F6A11

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-66399

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Plasminogen antibody (CL488-66399, Clone: 1F6A11 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL488-66399

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Plasminogen antibody (CL488-66399, Clone: 1F6A11 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-66399

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Plasminogen antibody (CL488-66399, Clone: 1F6A11 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66399 targets Plasminogen in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasminogen fusion protein Ag9938
• Full Name: plasminogen
• Calculated Molecular Weight: 810 aa, 91 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC060513
• Gene Symbol: Plasminogen
• Gene ID (NCBI): 5340
• RRID: AB_2919320
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.