Plasminogen Monoclonal antibody

Plasminogen Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1F6A11

Cat no : 66399-1-Ig

Synonyms

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin, 1F6A11

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Validation Data Gallery

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  WB analysis of human plasma using 66399-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66399-1-Ig (PLG Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66399-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66399-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66399-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

66399-1-Ig targets Plasminogen in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasminogen fusion protein Ag9938
• Full Name: plasminogen
• Calculated Molecular Weight: 810 aa, 91 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC060513
• Gene Symbol: Plasminogen
• Gene ID (NCBI): 5340
• RRID: AB_2881773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Publications

Species	Application	Title
mouse	WB	Angiogenesis; PDGFD switches on stem cell endothelial commitment.; Authors - Weisi Lu
human	WB	Aging (Albany NY); NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.; Authors - Pingping Ma
mouse	WB	Front Cell Dev Biol; Elevated Levels of miR-144-3p Induce Cholinergic Degeneration by Impairing the Maturation of NGF in Alzheimer's Disease.; Authors - Lan-Ting Zhou
mouse	IHC	Biomed Pharmacother; Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia.; Authors - Lina Wei
human	WB,IHC	Clin Transl Oncol; A prognostic gene signature for gastric cancer and the immune infiltration-associated mechanism underlying the signature gene, PLG; Authors - Hui Shi