Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody, PBS Only

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  WB analysis of NIH/3T3 using 80575-3-RR (same clone as 80575-3-PBS)

  NIH/3T3 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80575-3-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 80575-3-RR (same clone as 80575-3-PBS)

  PC-12 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80575-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 80575-3-RR (same clone as 80575-3-PBS)

  HEK-293 cells treated with 10% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80575-3-PBS in a different storage buffer formulation.

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 80575-3-PBS

Clone No.242048C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

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Product Information

80575-3-PBS targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P62753
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404)