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  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (80575-3-RR)

    WB analysis of NIH/3T3 using 80575-3-RR

    NIH/3T3 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

  • Western Blot (WB) analysis of PC-12 cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (80575-3-RR)

    WB analysis of PC-12 using 80575-3-RR

    PC-12 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (80575-3-RR)

    WB analysis using 80575-3-RR

    HEK-293 cells treated with 10% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80575-3-RR
Clone No.242048C2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Phos S6 Ribosomal protein (Ser240/244), Phos RPS6, Phos ribosomal protein S6, Phospho RPS6, Phospho-RPS6 (Ser240/244)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected in20% fetal bovine serum after serum free NIH/3T3 cells, 10% fetal bovine serum after serum free HEK-293 cells, 20% fetal bovine serum after serum free PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80575-3-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name ribosomal protein S6
Calculated Molecular Weight 29 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404)

Protocols

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 80575-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols